Gene Surgery Worksheet
An advanced genetics activity where students analyze a DNA sequence from the BCL11A enhancer to design a CRISPR-Cas9 sgRNA, predict cleavage outcomes, and evaluate repair pathways in the context of Sickle Cell Disease treatment.
Gene Surgery Answer Key
An instructor answer key for the Gene Surgery activity, providing correct DNA sequence locations, mechanistic explanations for Cas9 cleavage, and clinical context for BCL11A disruption.
Cas9 Mechanism Slides
A comprehensive visual presentation explaining the molecular mechanism of CRISPR-Cas9, including PAM recognition, R-loop formation, nuclease domain function, and the seed region's role in specificity.
Cas9 Mechanism Slides 2
A comprehensive visual presentation explaining the molecular mechanism of CRISPR-Cas9, including PAM recognition, R-loop formation, nuclease domain function, and the seed region's role in specificity. All text is optimized for legibility in a slide format.
Gene Surgery Lecture Slides
A specialized lecture slide deck designed to precede the Gene Surgery activity, providing clinical context on Sickle Cell Disease and a step-by-step molecular breakdown of the CRISPR-Cas9 mechanism.
Gene Surgery Lecture Slides 2
A specialized lecture slide deck designed to precede the Gene Surgery activity, providing clinical context on Sickle Cell Disease and a step-by-step molecular breakdown of the CRISPR-Cas9 mechanism. All text is at least 24px for classroom legibility.